Transcriptional effects of a positive feedback circuit in Drosophila melanogaster.

ABSTRACT:

Background

Synthetic systems that use positive feedback have been developed to control human disease vectors and crop pests. The tTAV system, which has been deployed in several insect species, relies on a positive feedback circuit that can be inhibited via dietary tetracycline. Although insects carrying tTAV fail to survive until adulthood in the absence of tetracycline, the exact reason for its lethality, as well as the transcriptomic effects of an active positive feedback circuit, remain unknown.

Results

We engineered the tTAV system in Drosophila melanogaster and investigated the effects of tTAV genome integration locus on the whole fly transcriptome during larval and adult life stages in four transgenic fly strains using gene expression microarrays. We found that while t

SUBMITTER: Bryk J 

PROVIDER: S-EPMC5746007 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature