Insulin modulates energy and substrate sensing and protein catabolism induced by chronic peritonitis in skeletal muscle of neonatal pigs.

ABSTRACT:

Background

Acute infection promotes skeletal muscle wasting and insulin resistance, but the effect of insulin on energy and substrate sensing in skeletal muscle of chronically infected neonates has not been studied.

Methods

Eighteen 2-d-old pigs underwent cecal ligation and puncture (CLP) or sham surgery (CON) to induce a chronic infection for 5 d. On d 5, pancreatic-substrate clamps were performed to attain fasting or fed insulin levels but to maintain glucose and amino acids in the fasting range. Total fractional protein synthesis rates (K_s), translational control mechanisms, and energy sensing and degradation signal activation were measured in longissimus dorsi muscle.

Results

In fasting conditions, CLP reduced K_s and sirtuin 1 (SIRT1) and inc

SUBMITTER: Manjarin R 

PROVIDER: S-EPMC5746053 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature