Eosinophil persistence in vivo and sustained viability ex vivo in response to respiratory challenge with fungal allergens.

ABSTRACT:

Background

Eosinophils are immunomodulatory leucocytes that contribute to the pathogenesis of Th2-driven asthma and allergic lung diseases.

Objective

Our goal was to identify unique properties of eosinophils recruited to the lungs and airways of mice in response to challenge with asthma-associated fungal allergens.

Methods

Mice were challenged intranasally on days 0, 3 and 6 with a filtrate of Alternaria alternata. Recruited eosinophils were enumerated in bronchoalveolar lavage fluid. Eosinophils were also isolated from lungs of mice sensitized and challenged with Aspergillus fumigatus and evaluated ex vivo in tissue culture.

Results

Eosinophils persist in the airways for several weeks in response to brief provocation with A. alternata in wild-type, Gm-csf- and

SUBMITTER: Geslewitz WE 

PROVIDER: S-EPMC5746458 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature