Membrane capacitance of thousands of single white blood cells.

ABSTRACT: As label-free biomarkers, the electrical properties of single cells are widely used for cell type classification and cellular status evaluation. However, as intrinsic cellular electrical markers, previously reported membrane capacitances (e.g. specific membrane capacitance Cspec and total membrane capacitance Cmem) of white blood cells were derived from tens of single cells, lacking statistical significance due to low cell numbers. In this study, white blood cells were first separated into granulocytes and lymphocytes by density gradient centrifugation and were then aspirated through a microfluidic constriction channel to characterize both Cspec and Cmem Thousands of granulocytes (ncell = 3327) and lymphocytes (ncell = 3302) from 10 healthy blood donors were characterized, resulting in Csp

SUBMITTER: Wang K 

PROVIDER: S-EPMC5746575 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature