Cell-Type Specific Metabolic Flux Analysis: A Challenge for Metabolic Phenotyping and a Potential Solution in Plants.

ABSTRACT: Stable isotope labelling experiments are used routinely in metabolic flux analysis (MFA) to determine the metabolic phenotype of cells and tissues. A complication arises in multicellular systems because single cell measurements of transcriptomes, proteomes and metabolomes in multicellular organisms suggest that the metabolic phenotype will differ between cell types. In silico analysis of simulated metabolite isotopomer datasets shows that cellular heterogeneity confounds conventional MFA because labelling data averaged over multiple cell types does not necessarily yield averaged flux values. A potential solution to this problem-the use of cell-type specific reporter proteins as a source of cell-type specific labelling data-is proposed and the practicality of implementing this strategy in t

SUBMITTER: Rossi MT 

PROVIDER: S-EPMC5746739 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature