NMR-Based Identification of Metabolites in Polar and Non-Polar Extracts of Avian Liver.

ABSTRACT: Metabolites present in liver provide important clues regarding the physiological state of an organism. The aim of this work was to evaluate a protocol for high-throughput NMR-based analysis of polar and non-polar metabolites from a small quantity of liver tissue. We extracted the tissue with a methanol/chloroform/water mixture and isolated the polar metabolites from the methanol/water layer and the non-polar metabolites from the chloroform layer. Following drying, we re-solubilized the fractions for analysis with a 600 MHz NMR spectrometer equipped with a 1.7 mm cryogenic probe. In order to evaluate the feasibility of this protocol for metabolomics studies, we analyzed the metabolic profile of livers from house sparrow (Passer domesticus) nestlings raised on two different diets: livers fro

SUBMITTER: Fathi F 

PROVIDER: S-EPMC5746741 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature