Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs.

ABSTRACT: The distribution of silver (Ag) into remote organs secondary to the application of Ag nanoparticles (Ag-NP) to the lung is still incompletely understood and was investigated in the rat with imaging methods. Dose-finding experiments were carried out with 50 nm- or 200 nm-sized polyvinyl pyrrolidine (PVP)-coated Ag-NP using alveolar macrophages in vitro and female rats, which received Ag-NP via intratracheal instillation. In the main study, we administered 37.5-300 µg per rat lung of the more toxic Ag50-PVP and assessed the broncho-alveolar lavage fluid (BALF) for inflammatory cells, total protein and fibronectin after three and 21 days. In parallel, lung tissue was analysed for DNA double-strand breaks and altered cell proliferation. While 75-150 µg Ag50-PVP per rat lung caused a reversible

SUBMITTER: Wiemann M 

PROVIDER: S-EPMC5746931 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature