Profiling the onset of somatic embryogenesis in Arabidopsis.

ABSTRACT:

Background

Totipotency is the ability of a cell to regenerate a whole organism. Plant somatic embryogenesis (SE) is a remarkable example of totipotency because somatic cells reverse differentiation, respond to an appropriate stimulus and initiate embryo development. Although SE is an ideal system to investigate de-differentiation and differentiation, we still lack a deep molecular understanding of the phenomenon due to experimental restraints.

Results

We applied the INTACT method to specifically isolate the nuclei of those cells undergoing SE among the majority of non-embryogenic cells that make up a callus. We compared the transcriptome of embryogenic cells to the one of proliferating callus cells. Our analyses revealed that embryogenic cells are transcriptionally rather th

SUBMITTER: Magnani E 

PROVIDER: S-EPMC5747089 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature