Multifaceted Role of BTLA in the Control of CD8⁺ T-cell Fate after Antigen Encounter.

ABSTRACT: Purpose: Adoptive T-cell therapy using autologous tumor-infiltrating lymphocytes (TIL) has shown an overall clinical response rate 40%-50% in metastatic melanoma patients. BTLA (B-and-T lymphocyte associated) expression on transferred CD8⁺ TILs was associated with better clinical outcome. The suppressive function of the ITIM and ITSM motifs of BTLA is well described. Here, we sought to determine the functional characteristics of the CD8⁺BTLA⁺TIL subset and define the contribution of the Grb2 motif of BTLA in T-cell costimulation.Experimental Design: We determined the functional role and downstream signal of BTLA in both human CD8⁺ TILs and mouse CD8⁺ T cells. Functional assays were used including single-cell analysis, rev

SUBMITTER: Ritthipichai K 

PROVIDER: S-EPMC5748156 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature