Cytokine signaling through Drosophila Mthl10 ties lifespan to environmental stress.

ABSTRACT: A systems-level understanding of cytokine-mediated, intertissue signaling is one of the keys to developing fundamental insight into the links between aging and inflammation. Here, we employed Drosophila, a routine model for analysis of cytokine signaling pathways in higher animals, to identify a receptor for the growth-blocking peptide (GBP) cytokine. Having previously established that the phospholipase C/Ca²⁺ signaling pathway mediates innate immune responses to GBP, we conducted a dsRNA library screen for genes that modulate Ca²⁺ mobilization in Drosophila S3 cells. A hitherto orphan G protein coupled receptor, Methuselah-like receptor-10 (Mthl10), was a significant hit. Secondary screening confirmed specific binding of fluorophore-tagged GBP to both S

SUBMITTER: Sung EJ 

PROVIDER: S-EPMC5748187 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature