Sld5 Ensures Centrosomal Resistance to Congression Forces by Preserving Centriolar Satellites.

ABSTRACT: The migration of chromosomes during mitosis is mediated primarily by kinesins that bind to the chromosomes and move along the microtubules, exerting pulling and pushing forces on the centrosomes. We report that a DNA replication protein, Sld5, localizes to the centrosomes, resisting the microtubular pulling forces experienced during chromosome congression. In the absence of Sld5, centriolar satellites, which normally cluster around the centrosomes, are dissipated throughout the cytoplasm, resulting in the loss of their known function of recruiting the centrosomal protein, pericentrin. We observed that Sld5-deficient centrosomes lacking pericentrin were unable to endure the CENP-E- and Kid-mediated microtubular forces that converge on the centrosomes during chromosome congression, resulting

SUBMITTER: Kaur M 

PROVIDER: S-EPMC5748466 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature