Characterization of genetic intratumor heterogeneity in colorectal cancer and matching patient-derived spheroid cultures.

ABSTRACT: Patient-derived in vitro cultures of colorectal cancer (CRC) may help guide treatment strategies prior to patient treatment. However, most previous studies have been performed on a single biopsy per tumor. The purpose of this study was to analyze multiple spatially distinct biopsies from CRCs and see how well intratumor heterogeneity (ITH) was recapitulated in matching patient-derived spheroids. Three to five biopsies were collected from six CRC tumors. Each biopsy was split in two; one half was used for spheroid culturing, while the other half was used for DNA and RNA purification. For two patients, lymph node metastases were analyzed. Somatic mutations were called from whole exome sequencing data. Each tumor contained mutations shared across all biopsies and spheroids, including major CR

SUBMITTER: Arnadottir SS 

PROVIDER: S-EPMC5748486 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature