A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef.

ABSTRACT: The orphan crop, Eragrostis tef, was subjected to controlled drought conditions to observe the physiological parameters and proteins changing in response to dehydration stress. Physiological measurements involving electrolyte leakage, chlorophyll fluorescence and ultra-structural analysis showed tef plants tolerated water loss to 50% relative water content (RWC) before adverse effects in leaf tissues were observed. Proteomic analysis using isobaric tag for relative and absolute quantification (iTRAQ) mass spectrometry and appropriate database searching enabled the detection of 5727 proteins, of which 211 proteins, including a number of spliced variants, were found to be differentially regulated with the imposed stress conditions. Validation of the iTRAQ dataset was done with selecte

SUBMITTER: Kamies R 

PROVIDER: S-EPMC5748567 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature