Development and Validation of an Ultrasensitive Procalcitonin Sandwich Immunoassay.

ABSTRACT: Procalcitonin (PCT) is well established as a highly specific biomarker for the detection of bacterial infections and sepsis. However, the currently available diagnostic tests are not able to detect very low or very early increases of PCT or even baseline levels in healthy individuals or patients with non-bacterial infections. In order to be able to detect these very low concentrations of PCT, a sandwich immunoassay was developed using high sensitivity Single Molecule Array technology (Simoa). The assay was thoroughly validated and applied to analyze human cerebrospinal fluid (CSF) and serum samples from patients with bacterial or viral meningitis as well as CSF, serum, and K2 EDTA plasma from healthy control subjects. A 50-fold increase in sensitivity compared to the current gold standard

SUBMITTER: Carcamo Yanez VA 

PROVIDER: S-EPMC5748597 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature