Using Human iPSC-Derived Neurons to Uncover Activity-Dependent Non-Coding RNAs.

ABSTRACT: Humans are arguably the most complex organisms present on Earth with their ability to imagine, create, and problem solve. As underlying mechanisms enabling these capacities reside in the brain, it is not surprising that the brain has undergone an extraordinary increase in size and complexity within the last few million years. Human induced pluripotent stem cells (hiPSCs) can be differentiated into many cell types that were virtually inaccessible historically, such as neurons. Here, we used hiPSC-derived neurons to investigate the cellular response to activation at the transcript level. Neuronal activation was performed with potassium chloride (KCl) and its effects were assessed by RNA sequencing. Our results revealed the involvement of long non-coding RNAs and human-specific genetic varian

SUBMITTER: Bitar M 

PROVIDER: S-EPMC5748719 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature