SelexGLM differentiates androgen and glucocorticoid receptor DNA-binding preference over an extended binding site.

ABSTRACT: The DNA-binding interfaces of the androgen (AR) and glucocorticoid (GR) receptors are virtually identical, yet these transcription factors share only about a third of their genomic binding sites and regulate similarly distinct sets of target genes. To address this paradox, we determined the intrinsic specificities of the AR and GR DNA-binding domains using a refined version of SELEX-seq. We developed an algorithm, SelexGLM, that quantifies binding specificity over a large (31-bp) binding site by iteratively fitting a feature-based generalized linear model to SELEX probe counts. This analysis revealed that the DNA-binding preferences of AR and GR homodimers differ significantly, both within and outside the 15-bp core binding site. The relative preference between the two factors can b

SUBMITTER: Zhang L 

PROVIDER: S-EPMC5749176 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature