SIDR: simultaneous isolation and parallel sequencing of genomic DNA and total RNA from single cells.

ABSTRACT: Simultaneous sequencing of the genome and transcriptome at the single-cell level is a powerful tool for characterizing genomic and transcriptomic variation and revealing correlative relationships. However, it remains technically challenging to analyze both the genome and transcriptome in the same cell. Here, we report a novel method for simultaneous isolation of genomic DNA and total RNA (SIDR) from single cells, achieving high recovery rates with minimal cross-contamination, as is crucial for accurate description and integration of the single-cell genome and transcriptome. For reliable and efficient separation of genomic DNA and total RNA from single cells, the method uses hypotonic lysis to preserve nuclear lamina integrity and subsequently captures the cell lysate using antibody-conjuga

SUBMITTER: Han KY 

PROVIDER: S-EPMC5749184 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature