Irf4-dependent CD103⁺CD11b⁺ dendritic cells and the intestinal microbiome regulate monocyte and macrophage activation and intestinal peristalsis in postoperative ileus.

ABSTRACT:

Objective

Postoperative ileus (POI), the most frequent complication after intestinal surgery, depends on dendritic cells (DCs) and macrophages. Here, we have investigated the mechanism that activates these cells and the contribution of the intestinal microbiota for POI induction.

Design

POI was induced by manipulating the intestine of mice, which selectively lack DCs, monocytes or macrophages. The disease severity in the small and large intestine was analysed by determining the distribution of orally applied fluorescein isothiocyanate-dextran and by measuring the excretion time of a retrogradely inserted glass ball. The impact of the microbiota on intestinal peristalsis was evaluated after oral antibiotic treatment.

Results

We found that Cd11c-Cre⁺ Irf4<

SUBMITTER: Pohl JM 

PROVIDER: S-EPMC5749346 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature