Identification of metastasis driver genes by massive parallel sequencing of successive steps of breast cancer progression.

ABSTRACT: Cancer results from alterations at essential genomic sites and is characterized by uncontrolled cell proliferation, invasion and metastasis. Identification of driver genes of metastatic progression is essential, as metastases, not primary tumors, are fatal. To gain insight into the mutational concordance between different steps of malignant progression we performed exome sequencing and validation with targeted deep sequencing of successive steps of malignant progression from pre-invasive stages to asynchronous distant metastases in six breast cancer patients. Using the ratio of non-synonymous to synonymous mutations, a surprisingly large number of cancer driver genes, ranging between 3 and 145, were estimated to confer a selective advantage in the studied primary tumors. We report a substa

SUBMITTER: Kroigard AB 

PROVIDER: S-EPMC5749725 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature