Macrophage conditioned medium promotes colorectal cancer stem cell phenotype via the hedgehog signaling pathway.

ABSTRACT:

Background

There is conflicting data on the role of macrophages in colorectal cancer (CRC); some studies have shown that macrophages can exert an anti-tumor effect whereas others show that macrophages are tumor promoting. We sought to determine the role of conditioned medium (CM) from macrophages, in particular classically activated macrophages, on the development of the CSC phenotype in CRC cells, which is believed to mediate tumor growth and chemoresistance.

Methods

Murine (CT26) and human (HCP-1) CRC cell lines were treated with CM from lipopolysaccharide (LPS)-activated murine macrophages. The CSC population was assessed using the sphere-forming assay and aldehyde dehydrogenase assay. Chemoresistance studies were performed using the MTT assay. CSC transcription factors a

SUBMITTER: Fan F 

PROVIDER: S-EPMC5749743 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature