MiR-34a regulates the invasive capacity of canine osteosarcoma cell lines.

ABSTRACT:

Background

Osteosarcoma (OSA) is the most common bone tumor in children and dogs; however, no substantial improvement in clinical outcome has occurred in either species over the past 30 years. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and play a fundamental role in cancer. The purpose of this study was to investigate the potential contribution of miR-34a loss to the biology of canine OSA, a well-established spontaneous model of the human disease.

Methodology and principal findings

RT-qPCR demonstrated that miR-34a expression levels were significantly reduced in primary canine OSA tumors and canine OSA cell lines as compared to normal canine osteoblasts. In canine OSA cell lines stably transduced with empty vector or pre-miR-34a lentiviral con

SUBMITTER: Lopez CM 

PROVIDER: S-EPMC5749745 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature