Consistent induction of chronic experimental autoimmune encephalomyelitis in C57BL/6 mice for the longitudinal study of pathology and repair.

ABSTRACT:

Background

While many groups use experimental autoimmune encephalomyelitis (EAE) as a model to uncover therapeutic targets and understand the pathology underlying multiple sclerosis (MS), EAE protocol variability introduces discrepancies in central nervous system (CNS) pathogenesis and clinical disease, limiting the comparability between studies and slowing much-needed translational research.

Optimized method

Here we describe a detailed, reliable protocol for chronic EAE induction in C57BL/6 mice utilizing two injections of myelin oligodendrocyte glycoprotein (35-55) peptide mixed with complete Freund's adjuvant and paired with pertussis toxin.

Results

The active MOG_35-55-EAE protocol presented here induces ascending paralysis in 80-100% of immunized mice.

SUBMITTER: Hasselmann JPC 

PROVIDER: S-EPMC5749979 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature