Characterizing highly dynamic conformational states: The transcription bubble in RNAP-promoter open complex as an example.

ABSTRACT: Bio-macromolecules carry out complicated functions through structural changes. To understand their mechanism of action, the structure of each step has to be characterized. While classical structural biology techniques allow the characterization of a few "structural snapshots" along the enzymatic cycle (usually of stable conformations), they do not cover all (and often fast interconverting) structures in the ensemble, where each may play an important functional role. Recently, several groups have demonstrated that structures of different conformations in solution could be solved by measuring multiple distances between different pairs of residues using single-molecule Förster resonance energy transfer (smFRET) and using them as constrains for hybrid/integrative structural modeling. However,

SUBMITTER: Lerner E 

PROVIDER: S-EPMC5750056 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature