Correlation of Circulating CD64⁺/CD163⁺ Monocyte Ratio and stroma/peri-tumoral CD163⁺ Monocyte Density with Human Papillomavirus Infected Cervical Lesion Severity.

ABSTRACT: HPV infected cervical cells secrete mediators that are gradually changed and have influence on infiltrating M2 phenotypic monocytes in cervical lesions. However, profiles of circulating immune cells in women with cervical lesions and M2 phenotypic monocyte activity in HPV infected cervical lesions are limited. This study aimed to investigate circulating monocyte populations correlated with M2 phenotype density and its activity in HPV infected cervical lesions. HPV DNA was investigated in cervical tissues using PCR. High risk HPV E6/E7 mRNA was detected using in situ hybridization. CD163 immunohistochemical staining was performed for M2 macrophage. CD163 and Arg1 mRNA expression were detected using real-time PCR. Circulating monocyte subpopulations were analyzed using flow cytometry. CD163

SUBMITTER: Swangphon P 

PROVIDER: S-EPMC5750203 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature