Sensitive mass spectrometric assay for determination of 15-deoxy-Δ^12,14-prostaglandin J₂ and its application in human plasma samples of patients with diabetes.

ABSTRACT: The determination of individual prostaglandins (PG) in humans is mainly performed in urine samples. The quantification of PGs in human plasma could improve the understanding of particular PG species under various physiological and pathological conditions. 15-Deoxy-Δ^12,14-prostaglandin J₂ (15d-PGJ₂) is a dehydrated downstream product of PGD₂ and is of high interest due to its recently discovered anti-inflammatory effects. Increasing availability of highly sensitive mass spectrometry allows the quantification of low abundant biomarkers like 15d-PGJ₂ in human plasma samples. Herein, a sensitive LC-MS/MS method for the determination of 15d-PGJ₂ was established. The method was validated according to the guidance of the American F

SUBMITTER: Morgenstern J 

PROVIDER: S-EPMC5750338 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature