Cloning of K26 Hydrophilic Antigen from Iranian Strain of Leishmania infantum.

ABSTRACT:

Background

Visceral leishmaniasis (VL) caused by Leishmania infantum is the most severe form of leishmaniasis in Iran, which causes a high mortality rate in the case of inaccurate diagnosis and treatment. This study aimed to clone of K26 gene from Iranian strain of L. infantum and register the sequencing results in Genbank to facilitate the preparation a new K26 antigen for the detection of L. infantum infection.

Methods

L. infantum was obtained from an infected domestic dog in Meshkin-Shahr area from northwestern Iran in 2015. Canine visceral leishmaniasis was confirmed by direct agglutination test (DAT), rK39 dipstick and parasitological methods. L. infantum was confirmed by N-acetyl glucosamine -1-phosphate transferase (nagt)-PCR and its

SUBMITTER: Hosseini Farash BR 

PROVIDER: S-EPMC5750347 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature