Neural Differentiation Is Inhibited through HIF1α/β-Catenin Signaling in Embryoid Bodies.

ABSTRACT: Extensive research in the field of stem cells and developmental biology has revealed evidence of the role of hypoxia as an important factor regulating self-renewal and differentiation. However, comprehensive information about the exact hypoxia-mediated regulatory mechanism of stem cell fate during early embryonic development is still missing. Using a model of embryoid bodies (EBs) derived from murine embryonic stem cells (ESC), we here tried to encrypt the role of hypoxia-inducible factor 1α (HIF1α) in neural fate during spontaneous differentiation. EBs derived from ESC with the ablated gene for HIF1α had abnormally increased neuronal characteristics during differentiation. An increased neural phenotype in Hif1α-/- EBs was accompanied by the disruption of β-catenin signaling together with

SUBMITTER: Vecera J 

PROVIDER: S-EPMC5750467 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature