O-Glycome Beam Search Arrays for Carbohydrate Ligand Discovery.

ABSTRACT: O-glycosylation is a post-translational modification of proteins crucial to molecular mechanisms in health and disease. O-glycans are typically highly heterogeneous. The involvement of specific O-glycan sequences in many bio-recognition systems is yet to be determined because of a lack of efficient methodologies. We describe here a targeted microarray approach: O-glycome beam search that is both robust and efficient for O-glycan ligand-discovery. Substantial simplification of the complex O-glycome profile and facile chromatographic resolution is achieved by arraying O-glycans as branches, monitoring by mass spectrometry, focusing on promising fractions, and on-array immuno-sequencing. This is orders of magnitude more sensitive than tr

SUBMITTER: Li Z 

PROVIDER: S-EPMC5750842 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature