Physical mapping and candidate gene prediction of fertility restorer gene of cytoplasmic male sterility in cotton.

ABSTRACT:

Background

Cytoplasmic male sterility (CMS) is a maternally inherited trait failing to produce functional pollen. It plays a pivotal role in the exploitation of crop heterosis. The specific locus amplified fragment sequencing (SLAF-seq) as a high-resolution strategy for the identification of new SNPs on a large-scale is gradually applied for functional gene mining. The current study combined the bulked segregant analysis (BSA) with SLAF-seq to identify the candidate genes associated with fertility restorer gene (Rf) in CMS cotton.

Methods

Illumina sequencing systematically investigated the parents. A segregating population comprising of 30 + 30 F₂ individuals was developed using 3096A (female parent) as sterile and 866R (male parent) as a restorer. The original da

SUBMITTER: Zhao C 

PROVIDER: S-EPMC5751606 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature