Improving molecular tools for global surveillance of measles virus.

ABSTRACT:

Background

The genetic characterization of wild-type measles viruses plays an important role in the description of viral transmission pathways and the verification of measles elimination. The 450 nucleotides that encode the carboxyl-terminus of the nucleoprotein (N-450) are routinely sequenced for genotype analysis.

Objectives

The objectives of this study were to develop improved primers and controls for RT-PCR reactions used for genotyping of measles samples and to develop a method to provide a convenient, safe, and inexpensive means to distribute measles RNA for RT-PCR assays and practice panels.

Study design

A newly designed, genetically defined synthetic RNA and RNA isolated from cells infected with currently circulating genotypes were used to compare the sensitiv

SUBMITTER: Bankamp B 

PROVIDER: S-EPMC5751696 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature