Functional characterization of chitin-binding lectin from Solanum integrifolium containing anti-fungal and insecticidal activities.

ABSTRACT:

Background

Along with the rapid development of glycomic tools, the study of lectin-carbohydrate interactions has expanded, opening the way for applications in the fields of analytic, diagnostic, and drug delivery. Chitin-binding lectins (CBLs) play roles in immune defense against chitin-containing pathogens. CBLs from species of the Solanaceae family, such as tomato, potato and jimsonweed, display different binding specificities to sugar chains containing poly-N-acetyllactosamine.

Results

In this report, CBLs from Solanum integrifolium were isolated by ion exchange chromatography. The fractions showed hemagglutination activity (HA). The recombinant CBL in the 293F cell culture supernatant was able to inhibit the growth of Rhizoctonia solani and Colletotrichum gloeosporioide.

SUBMITTER: Chen CS 

PROVIDER: S-EPMC5751800 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature