Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo.

ABSTRACT: Structural changes in the retina are common manifestations of ophthalmic diseases. Optical coherence tomography (OCT) enables their identification in vivo-rapidly, repetitively, and at a high resolution. This protocol describes OCT imaging in the mouse retina as a powerful tool to study optic neuropathies (OPN). The OCT system is an interferometry-based, non-invasive alternative to common post mortem histological assays. It provides a fast and accurate assessment of retinal thickness, allowing the possibility to track changes, such as retinal thinning or thickening. We present the imaging process and analysis with the example of the Opa1^delTTAG mouse line. Three types of scans are proposed, with two quantification methods: standard and homemade calipers. The latter is best for u

SUBMITTER: Jagodzinska J 

PROVIDER: S-EPMC5752311 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature