Hybrid Structured Illumination Expansion Microscopy Reveals Microbial Cytoskeleton Organization.

ABSTRACT: Recently developed tissue-hydrogel methods for specimen expansion now enable researchers to perform super-resolution microscopy with ∼65 nm lateral resolution using ordinary microscopes, standard fluorescent probes, and inexpensive reagents. Here we use the combination of specimen expansion and the optical super-resolution microscopy technique structured illumination microscopy (SIM) to extend the spatial resolution to ∼30 nm. We apply this hybrid method, which we call ExSIM, to study the cytoskeleton of the important human pathogen Giardia lamblia including the adhesive disc and flagellar axonemes. We determined the localization of two recently identified disc-associated proteins, including DAP86676 , which localizes to disc microribbons, and the functionally unknown DAP16263 , which prim

SUBMITTER: Halpern AR 

PROVIDER: S-EPMC5752594 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature