Influence of TBX21 T-1993C variant on autoimmune hepatitis development by Yin-Yang 1 binding.

ABSTRACT:

Aim

To investigated the mechanism of the association between the TBX21 T-1993C promoter polymorphism and autoimmune hepatitis type 1 (AIH-1) development.

Methods

In vivo, In vivo, and reporter analyses were performed to determine the function of transcription factors binding to the T-1993C element of the TBX21 promoter in human CD4⁺ T and B cell lines. Flow cytometry and quantitative real-time PCR were used to analyze T-box transcription factor (T-bet) and interferon-γ (IFN-γ) expressions in CD4⁺ T cells, B cells and monocytes from the peripheral blood of AIH-1 patients including 5-1993TC and 15-1993TT genotype carriers, and healthy controls including 10-1993TC and 25-1993TT genotype carriers. Furthermore, a range of biochemical indices wa

SUBMITTER: Sun W 

PROVIDER: S-EPMC5752710 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature