Colony-PCR Is a Rapid Method for DNA Amplification of Hyphomycetes.

ABSTRACT: Fungal pure cultures identified with both classical morphological methods and through barcoding sequences are a basic requirement for reliable reference sequences in public databases. Improved techniques for an accelerated DNA barcode reference library construction will result in considerably improved sequence databases covering a wider taxonomic range. Fast, cheap, and reliable methods for obtaining DNA sequences from fungal isolates are, therefore, a valuable tool for the scientific community. Direct colony PCR was already successfully established for yeasts, but has not been evaluated for a wide range of anamorphic soil fungi up to now, and a direct amplification protocol for hyphomycetes without tissue pre-treatment has not been published so far. Here, we present a colony PCR technique

SUBMITTER: Walch G 

PROVIDER: S-EPMC5753074 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature