Genotype and biotype of invasive Anopheles stephensi in Mannar Island of Sri Lanka.

ABSTRACT:

Background

Anopheles stephensi, the major vector of urban malaria in India, was recently detected for the first time in Sri Lanka in Mannar Island on the northwestern coast. Since there are different biotypes of An. stephensi with different vector capacities in India, a study was undertaken to further characterise the genotype and biotype of An. stephensi in Mannar Island.

Methods

Mosquito larvae were collected in Pesalai village in Mannar and maintained in the insectary until adulthood. Adult An. stephensi were identified morphologically using published keys. Identified adult An. stephensi were molecularly characterized using two mitochondrial (cox1 and cytb) and one nuclear (ITS2) markers. Their PCR-amplified target fragments were sequenced and checked against available se

SUBMITTER: Surendran SN 

PROVIDER: S-EPMC5753456 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature