IMMUNEPOTENT CRP induces cell cycle arrest and caspase-independent regulated cell death in HeLa cells through reactive oxygen species production.

ABSTRACT:

Background

Regulated cell death (RCD) is a mechanism by which the cell activates its own machinery to self-destruct. RCD is important for the maintenance of tissue homeostasis and its deregulation is involved in diseases such as cervical cancer. IMMUNEPOTENT CRP (I-CRP) is a dialyzable bovine leukocyte extract that contains transfer factors and acts as an immunomodulator, and can be cytotoxic to cancer cell lines and reduce tumor burden in vivo. Although I-CRP has shown to improve or modulate immune response in inflammation, infectious diseases and cancer, its widespread use has been limited by the absence of conclusive data on the molecular mechanism of its action.

Methods

In this study we analyzed the mechanism by which I-CRP induces cytotoxicity in HeLa cells. We assessed

SUBMITTER: Martinez-Torres AC 

PROVIDER: S-EPMC5753472 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature