Biopanning of polypeptides binding to bovine ephemeral fever virus G₁ protein from phage display peptide library.

ABSTRACT:

Background

The bovine ephemeral fever virus (BEFV) glycoprotein neutralization site 1 (also referred as G₁ protein), is a critical protein responsible for virus infectivity and eliciting immune-protection, however, binding peptides of BEFV G₁ protein are still unclear. Thus, the aim of the present study was to screen specific polypeptides, which bind BEFV G₁ protein with high-affinity and inhibit BEFV replication.

Methods

The purified BEFV G₁ was coated and then reacted with the M13-based Ph.D.-7 phage random display library. The peptides for target binding were automated sequenced after four rounds of enrichment biopanning. The amino acid sequences of polypeptide displayed on positive clones were deduced and the affinity of positi

SUBMITTER: Hou P 

PROVIDER: S-EPMC5753476 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature