Shuang-Huang-Lian prevents basophilic granulocyte activation to suppress Th2 immunity.

ABSTRACT:

Background

Basophilic granulocytes (BGs) not only initiate the induction of Th2 cell differentiation, but also amplify the ongoing Th2 response. Shuang-Huang-Lian (SHL) is clinically used for relieving type I hypersensitivity by continuous treatment for several weeks.

Methods

ELISA, flow cytometry, magnetic activated cell sorting, isoelectric precipitation, hybridoma technique, transfection and luciferase reporter assay were used in this study. The statistical analysis was performed using a one-way ANOVA.

Results

Our recently published study demonstrated that SHL exerted a remarkable effect on mast cell stabilization. Herein, we sought to elucidate the effect of SHL on shrimp tropomyosin (ST)-induced Th2 immunity and its underlying mechanisms. The obtained data showed

SUBMITTER: Fei Q 

PROVIDER: S-EPMC5753509 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature