Protein Moonlighting Revealed by Noncatalytic Phenotypes of Yeast Enzymes.

ABSTRACT: A single gene can partake in several biological processes, and therefore gene deletions can lead to different-sometimes unexpected-phenotypes. However, it is not always clear whether such pleiotropy reflects the loss of a unique molecular activity involved in different processes or the loss of a multifunctional protein. Here, using Saccharomyces cerevisiae metabolism as a model, we systematically test the null hypothesis that enzyme phenotypes depend on a single annotated molecular function, namely their catalysis. We screened a set of carefully selected genes by quantifying the contribution of catalysis to gene deletion phenotypes under different environmental conditions. While most phenotypes were explained by loss of catalysis, slow growth was readily rescued by a catalytically i

SUBMITTER: Espinosa-Cantu A 

PROVIDER: S-EPMC5753873 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature