Regulation of Intracellular Calcium by Endoplasmic Reticulum Proteins in Small Intestinal Interstitial Cells of Cajal.

ABSTRACT:

Background/aims

We investigated the role of representative endoplasmic reticulum proteins, stromal interaction molecule 1 (STIM1), and store-operated calcium entry-associated regulatory factor (SARAF) in pacemaker activity in cultured interstitial cells of Cajal (ICCs) isolated from mouse small intestine.

Methods

The whole-cell patch clamp technique applied for intracellular calcium ions ([Ca²⁺]_i) analysis with STIM1 or SARAF overexpressed cultured ICCs from mouse small intestine.

Results

In the current-clamping mode, cultured ICCs displayed spontaneous pacemaker potentials. External carbachol exposure produced tonic membrane depolarization in the current-clamp mode, which recovered within a few seconds into normal pacemaker potentials. In STIM1-

SUBMITTER: Park CG 

PROVIDER: S-EPMC5753911 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature