Intracellular water preexchange lifetime in neurons and astrocytes.

ABSTRACT:

Purpose

To determine the intracellular water preexchange lifetime, τ_i , the "average residence time" of water, in the intracellular milieu of neurons and astrocytes. The preexchange lifetime is important for modeling a variety of MR data sets, including relaxation, diffusion-sensitive, and dynamic contrast-enhanced data sets.

Methods

Herein, τ_i in neurons and astrocytes is determined in a microbead-adherent, cultured cell system. In concert with thin-slice selection, rapid flow of extracellular media suppresses extracellular signal, allowing determination of the transcytolemmal-exchange-dominated, intracellular T₁ . With this knowledge, and that of the intracellular T₁ in the absence of exchange, τ_i can be derived.

Re

SUBMITTER: Yang DM 

PROVIDER: S-EPMC5754269 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature