CRTC2 promotes non-small cell lung cancer A549 migration and invasion in vitro.

ABSTRACT:

Background

CRTC2 is highly expressed in lung cancer and contributes to lung cancer pathogenesis; however, whether CRTC2 promotes lung cancer metastasis remains unknown. In the present study, we investigated the role of CRTC2 in lung cancer metastasis in vitro.

Methods

CRTC2 stable knockdown of lung cancer cell A549 was generated with small hairpin RNA and confirmed by quantitative reverse transcription-PCR and Western blot. Wound healing and invasion transwell assays were performed to explore migration and invasion activity, and Western blot was conducted to detect the expression of related proteins.

Results

Suppression of CRTC2 significantly inhibited A549 cell migration and invasion in vitro. Mechanistic studies showed that knockdown of CRTC2 greatly downregulated M

SUBMITTER: Shi S 

PROVIDER: S-EPMC5754302 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature