Electrostatic confinement and manipulation of DNA molecules for genome analysis.

ABSTRACT: Very large DNA molecules enable comprehensive analysis of complex genomes, such as human, cancer, and plants because they span across sequence repeats and complex somatic events. When physically manipulated, or analyzed as single molecules, long polyelectrolytes are problematic because of mechanical considerations that include shear-mediated breakage, dealing with the massive size of these coils, or the length of stretched DNAs using common experimental techniques and fluidic devices. Accordingly, we harness analyte "issues" as exploitable advantages by our invention and characterization of the "molecular gate," which controls and synchronizes formation of stretched DNA molecules as DNA dumbbells within nanoslit geometries. Molecular gate geometries comprise micro- and nanoscale features d

SUBMITTER: Kounovsky-Shafer KL 

PROVIDER: S-EPMC5754773 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature