Metagenomic deep sequencing of aqueous fluid detects intraocular lymphomas.

ABSTRACT:

Introduction

Currently, the detection of pathogens or mutations associated with intraocular lymphomas heavily relies on prespecified, directed PCRs. With metagenomic deep sequencing (MDS), an unbiased high-throughput sequencing approach, all pathogens as well as all mutations present in the host's genome can be detected in the same small amount of ocular fluid.

Methods

In this cross-sectional case series, aqueous fluid samples from two patients were submitted to MDS to identify pathogens as well as common and rare cancer mutations.

Results

MDS of aqueous fluid from the first patient with vitreal lymphoma revealed the presence of both Epstein-Barr virus (HHV-4/EBV) and human herpes virus 8 (HHV-8) RNA. Aqueous fluid from the second patient with intraocular B-cell lymph

SUBMITTER: Gonzales J 

PROVIDER: S-EPMC5754869 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature