Systematic analysis of the molecular mechanism of microRNA-124 in hepatoblastoma cells.

ABSTRACT: The present study aimed to identify the molecular mechanisms of microRNA-124 (miRNA-124/miR-124) in hepatoblastoma. The GSE6207 microarray dataset, obtained from the Gene Expression Omnibus database, included samples extracted from HepG2 cells transfected with miR-124 duplex (the experimental group) or negative control (the control group) at 4, 8, 16, 24, 32, 72 and 120 h after transfection. Differentially expressed genes (DEGs) were screened between the two groups. miR-124 activity was inferred based on the expression of its target genes. The mRNAs targeted by miR-124 were predicted and a miR-124-target mRNA network was constructed. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed for the target genes. The number of DEGs was highest

SUBMITTER: Wang G 

PROVIDER: S-EPMC5754889 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature