Coordinated expression and genetic polymorphisms in Grainyhead-like genes in human non-melanoma skin cancers.

ABSTRACT:

Background

The Grainyhead-like (GRHL) transcription factors have been linked to many different types of cancer. However, no previous study has attempted to investigate potential correlations in expression of different GRHL genes in this context. Furthermore, there is very little information concerning damaging mutations and/or single nucleotide polymorphisms in GRHL genes that may be linked to cancer.

Methods

DNA and RNA were extracted from human non-melanoma skin cancers (NMSC) and adjacent normal tissues (n = 33 pairs of samples). The expression of GRHL genes was measured by quantitative real time PCR. Regulation of GRHL expression by miRNA was studied using cell transfection methods and dual-luciferase reporter system. Targeted deep sequencing of GRHL genes in tumor sampl

SUBMITTER: Kikulska A 

PROVIDER: S-EPMC5755140 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature