Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity.

ABSTRACT: Human norovirus exacts considerable public health and economic losses worldwide. Emerging in vitro cultivation advances are not yet applicable for routine detection of the virus. The current detection and quantification techniques, which rely primarily on nucleic acid amplification, do not discriminate infectious from non-infectious viral particles. The purpose of this article is to present specific details on recent advances in techniques used together in order to acquire further information on the infectivity status of viral particles. One technique involves assessing binding of a norovirus ssDNA aptamer to capsids. Aptamers have the advantage of being easily synthesized and modified, and are inexpensive and stable. Another technique, dynamic light scattering (DLS), has the advantage of

SUBMITTER: Moore MD 

PROVIDER: S-EPMC5755425 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature