Evaluation of a method to measure HHV-6B infection in vitro based on cell size.

ABSTRACT:

Background

Human herpesvirus 6 (HHV-6A and HHV-6B) infection of cell cultures can be measured by different methods, including immunofluorescence microscopy, flow cytometry, or quantification of virus DNA by qPCR. These methods are reliable and sensitive but require long processing times and can be costly. Another method used in the field relies on the identification of enlarged cells in the culture; this method requires little sample processing and is relatively fast. However, visual inspection of cell cultures can be subjective and it can be difficult to establish clear criteria to decide if a cell is enlarged. To overcome these issues, we explored a method to monitor HHV-6B infections based on the systematic and objective measurement of the size of cells using an imaging-based au

SUBMITTER: Becerra-Artiles A 

PROVIDER: S-EPMC5755443 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature