Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid.

ABSTRACT: This protocol describes a method developed to identify endogenous peptides in human cerebrospinal fluid (CSF). For this purpose, a previously developed method based on molecular weight cut-off (MWCO) filtration and mass spectrometric analysis was combined with an offline high-pH reverse phase HPLC pre-fractionation step. Secretion into CSF is the main pathway for removal of molecules shed by cells of the central nervous system. Thus, many processes in the central nervous system are reflected in the CSF, rendering it a valuable diagnostic fluid. CSF has a complex composition, containing proteins that span a concentration range of 8 - 9 orders of magnitude. Besides proteins, previous studies have also demonstrated the presence of a large number of endogenous peptides. While less extensively

SUBMITTER: Hansson KT 

PROVIDER: S-EPMC5755527 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature